Texas Bacterial Source Tracking Program

The Need

There are 273 bacterially impaired water bodies in Texas. Identifying and assessing sources of these bacteria — E. coli, Enterococcus, fecal coliforms — is critical to properly determining risk to water recreation, developing effective watershed restoration strategies such as watershed protection plans (WPPs) and total maximum daily loads (TMDLs), and efficiently targeting management measures.

Options for Bacterial Source Identification and Advantages of BST

Bacterial sources are extensive and include all birds, wildlife, livestock, pets and humans. Source surveys, computer models and bacterial source tracking (BST) are the primary methods for identifying predominant bacterial sources in impaired waters. BST offers distinct advantages over computer modeling and source surveys. Source surveys, which estimate the numbers and distributions of animals and humans in a watershed, are not capable of assessing most wildlife species or how bacteria from sources are transported to the impaired waters. Computer modeling addresses the issues with transport; however, because wildlife populations are rarely known, models are unable to adequately assess contributions from wildlife. BST is able to evaluate wildlife, along with other major sources, and the impacts of transport because BST uses instream water samples for its assessment. However, best results are generally achieved when used in conjunction with the other methods.

BST Technologies

The premise behind BST is that DNA fingerprinting and other molecular targets can identify bacterial strains specific to each animal species since each species has different diets and digestive system conditions that select for distinct strains of bacteria. This distinction allows the original source of the fecal contamination to be identified.

Numerous BST methods are available. Based on a multi-year study initiated in 2002, Texas selected the two-method approach using ERIC-PCR and RiboPrinting, as this approach was found to be the most accurate and cost-effective. Because it provides a direct link with water quality standards, E. coli is used as the target bacteria. Using these methods required development of a DNA fingerprint library.

About the Texas BST Library

The Texas BST Library currently contains more than 1,700 E. coli isolates obtained from more than 1,500 different domestic sewage, wildlife, livestock and pet fecal samples. These isolates, which represent more than 50 animal subclasses, were selected after screening more than 6,700 E. coli from more than a dozen different studies throughout Texas.

Currently, Dr. George Di Giovanni at The University of Texas Health Science Center at Houston (UTHealth) School of Public Health in El Paso and Dr. Terry Gentry at the Texas A&M University Soil and Aquatic Microbiology Laboratory oversee and maintain the Texas BST Library, continuously testing its accuracy and refining its composition.

BST has been tremendously helpful in identifying significant bacterial sources throughout Texas. The state BST laboratories can provide guidance and assistance with performing BST for watersheds.


Location of known source fecal sample collection efforts supporting the development of the Texas E. coli BST Library.


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